Home > Basics of GM Foods > Types of Techniques Used to Genetically Modify Food

Types of Techniques Used to Genetically Modify Food

By: Ian Murnaghan BSc (hons), MSc - Updated: 19 Sep 2018 | comments*Discuss
Gm Food Techniques Experiments Food Safe

While genetically modified (GM) foods are a controversial subject given the questions about their effect on the environment and human health, little attention is given to the techniques and experiments used to modify these foods. These techniques are important to understand because they affect the final organism that results. In fact, the development of these techniques and experiments is quite fascinating given how rapidly the biotechnology field has advanced over the last two decades. Ensuring that the techniques are safe and that food is safe as well are important considerations.

Bacterial Carriers

Bacterial carriers can be very effective for delivering DNA. Typically, a bacterium would be prepped in a solution that makes the cell walls extremely porous. The chosen gene would then be inserted into a plasmid and put in the solution. After heating the solution, the plasmid is able to 'merge' with the bacterium and show the new gene. Once the genetically modified bacterium recovers and grows, it can make additional replicates of the new gene. After infecting the targeted plant, it can deliver the plasmid and the new gene.

Calcium Phosphate Precipitation

In this biotechnology technique, the chosen DNA would be exposed to calcium phosphate, which results in the creation of miniscule granules. The targeted cells react to the granules by essentially 'swarming' them and ingesting them, thereby facilitating the granule release of DNA and the subsequent delivery to the host's nuclei and chromosomes.

Using Electroporation To Create GM Organisms

In electroporation, the prepped target cells are saturated in a solution with the chosen DNA. A brief but strong electric shock is transmitted through the solution, causing little tears in the walls of the cells. This allows for the new genetic material to penetrate the nuclei. Afterwards, the cells are put in a different solution that coaxes the repair of their walls, which works to 'trap' the DNA of the donor in the cell. The chosen DNA becomes joined with the host chromosomes to give the host this new gene.

Biolistics For GM Technology

This technique uses the chosen DNA to attach it to tiny gold particles. The particles – now 'carrying' DNA – are forced into the target cells using an intense burst of gas.

Gene Silencing Technique

With GM techniques, they are sometimes used to remove a gene that is responsible for an undesirable trait. When gene silencing is used, the gene that is responsible for this trait will first be identified in the organism. Then, another copy of the gene is attached but in the other direction, which prevents the expression of that trait. For instance, an allergen that triggers an allergic reaction in humans could be 'silenced' in this manner.

Gene Splicing

With this GM technique, biotechnologists can modify DNA, and then insert it into target host cells to allow for genes and resulting traits to be modified. An enzyme is then used to fuse the newly added gene into the chromosome.

Using A Viral Carrier

A virus can make an effective carrier for modifying an organism. The virus chosen will be one that does not cause any kind of disease or death. Through the addition of the chosen DNA to the virus genome, the virus can infect the target. Once the virus invades the cell and makes copies of itself, the chosen DNA can be added to the targeted cell.

There are other techniques such as lipofection or microinjection, which show how vast the array of techniques is for modifying an organism. Care always has to be taken to choose the most effective technique to produce the desired result. While GM foods will likely continue to be controversial, the techniques that allow for their creation will also continue to advance at a rapid pace.

You might also like...
Share Your Story, Join the Discussion or Seek Advice..
I support the introduction of GM into crops but scientist behind this technology should be extra careful when breeding plant/seeds so as the whole world not suffer as it happening in USA during the early period of commercial maize Hybrids seeds was introduce in 1920's to early 30's. More education in the form of scholarships should be offer to us (who we support GM in crops) so as to help sensitize our colleagues about GM food.
Agya Amoako - 8-Jun-14 @ 10:19 PM
Share Your Story, Join the Discussion or Seek Advice...
(never shown)
(never shown)
(never shown)
(never shown)
Enter word:
Latest Comments
  • achakar andrew
    Re: Ethical Concerns and GM Foods
    My ethical concern is equity between those who use GMOs and those using organic .can those who use gmos be better than others who…
    9 April 2019
  • Dr.Farncer
    Re: Ethical Concerns and GM Foods
    I think GMOs are stupid we need to use what we have available and stop killing the human race
    14 March 2019
  • supiegy
    Re: Ethical Concerns and GM Foods
    loved it for school was amazing!!! but there not very ethical and are more environmental rather than ethical. but thx
    13 March 2019
  • sadfdfwe
    Re: Ethical Concerns and GM Foods
    There are a number of ethical concerns over genetically modified (GM) foods and these have all affected public support of the…
    13 March 2019
  • big bouncing baby bo
    Re: Ethical Concerns and GM Foods
    what if gmos are actually gmod mods in disguise?!!?!?
    25 February 2019
  • Reverend Upp
    Re: Fact Sheet: Pros vs Cons
    GMO's are unsanctioned by our true god, the flying spaghetti monster! Truly unblessed
    20 February 2019
  • LuiG97
    Re: Short-term Effects of GM Foods
    Shanqueesha, you must be real autistic or something. You are part of the 70%ish people who think GMOs are bad to the people. You…
    26 November 2018
    Re: Making Crops More Resilient
    Hi my name is not Joey Sager and I would love to tell you how much I love (hate) this website :)
    1 November 2018
  • N/A
    Re: Ethical Concerns and GM Foods
    Although the author does not have any Works Cited or Resources, he does have a valid point concerning GMOs. It is a relatively…
    23 October 2018
  • Rick and Morty
    Re: Ethical Concerns and GM Foods
    this site doesn't use any sources or researches or studies, non of this has any supporting point provided, just some person…
    22 August 2018